ICOSL/CHO
CBP74068
詢 價(jià)
索取Protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫(kù)
| I. Background | |
| ICOS 是一種在 T 細(xì)胞活化后才被誘導(dǎo)表達(dá)的共刺激受體，而 ICOSL 是其配體，主要 組成性表達(dá)于 B 細(xì)胞和抗原呈遞細(xì)胞表面。兩者的核心作用機(jī)理在于，當(dāng)活化的 T 細(xì)胞 （特別是濾泡輔助性 T 細(xì)胞）遷移至淋巴濾泡并與 B 細(xì)胞相遇時(shí)，ICOS 與 ICOSL 的結(jié)合 為 T 細(xì)胞提供了關(guān)鍵的共刺激信號(hào)，這主要通過(guò)激活 PI3K 等下游通路，驅(qū)動(dòng) T 細(xì)胞增殖、 存活及產(chǎn)生細(xì)胞因子（如 IL-21），從而精確調(diào)控生發(fā)中心反應(yīng)，最終促進(jìn) B 細(xì)胞進(jìn)行抗 體親和力成熟與類別轉(zhuǎn)換，產(chǎn)生高效體液免疫。 | |
| II. Description | |
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ICOSL CHO 細(xì)胞，在 CHO-K1 細(xì)胞的基礎(chǔ)上，穩(wěn)定表達(dá)人 ICOSL。 |
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| III. Introduction | |
| Host Cell: | CHO |
| Expressed gene: | ICOSL |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+500μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Description of Host Cell Line | |
| Organism: | Cricetulus griseus, hamster, Chinese |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
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Figure 1.Recombinant ICOSL/CHO constitutively expressing ICOSL. |
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| Binding Assay,FACS |
